Hideaki Moriyama

Associate Professor Profile Image
Associate Professor Biological Sciences hmoriyama2@unl.edu 402-472-5367 Manter Hall 243

Research Interests

The H. Moriyama Lab is interested in the structure and function of proteins that trigger biological processes, in order to better understand and treat metabolic and behavior disorders. Our research involves the interdisciplines of mathematical biology, biophysics, chemical biology, molecular and structural biology plus bioinformatics. Our passion is to reconstruct critical signaling nodes how these drive responses based on the substance oriented science.

Protein Allostery and Modulators
We are interested in the internal protein atomic network. How protein preserves the energy to give allosteric motion upon ligand binding? Can we discover the method to cancel the preserved energy to be used in the protein motions? We are using the time-resolved protein crystallography and the small angle scattering using free electron laser, X-ray, and neutron as probes.

Chemoreceptor Signaling Pathways
We are working to communicate with many chemoreceptor in animals, including insects and monkeys. Which chemical compounds are chemoreceptor targets? How are thy recognized by the chemoreceptor? Can we extract the voice of chemoreceptors? Can we change the tone in the therapeutic purpose?

Temperature Deriven Evolution
We are approaching the problem how enzymes’ optimal temperature and pH defined. Can we address enzymes from organisms found by the antarctic drilling as the most evolved version of that from the hot spring organisms? What are the fine tuning methods nature used to fit the optimal temperature and pH in the process of adaptation? How large is the functional merging do organisms allow?

Recent Publications

  • Cheviron, Z. A., Natarajan, C., Projecto-Garcia, J., Eddy, D. K., Jones, J., Carling, M. D., Witt, C. C., Moriyama, H., Weber, R. E., Fago, A., and Storz, J. F. (2014). Integrating Evolutionary and Functional Tests of Adaptive Hypotheses: A Case Study of Altitudinal Differentiation in Hemoglobin Function in an Andean Sparrow, Zonotrichia capensis. Mol Biol Evol 31, 2948-2962.
  • Tufts, D. M., Natarajan, C., Revsbech, I. G., Projecto-Garcia, J., Hoffmann, F. G., Weber, R. E., Fago, A., Moriyama, H., and Storz, J. F. (2014). Epistasis Constrains Mutational Pathways of Hemoglobin Adaptation in High-Altitude Pikas. Mol Biol Evol
  • Joana Projecto-Garcia, Chandrasekhar Natarajan, Hideaki Moriyama, Roy E. Weber, Angela Fago, Zachary A. Cheviron, Robert Dudley, Jimmy A. McGuire, Christopher C. Witt, and Jay F. Storz (2013) Repeated elevational transitions in hemoglobin function during the evolution of Andean hummingbirds. PNAS 2013 ; published ahead of print December 2, 2013, doi:10.1073/pnas.1315456110      
  • Revsbech IG, Tufts DM, Projecto-Garcia J, Moriyama H, Weber RE, Storz JF, Fago A. Hemoglobin function and allosteric regulation in semi-fossorial rodents (family Sciuridae) with different altitudinal ranges. J. Exp. Biol. 2013:16, 4264-71.
  • Shah N, Gaupp R, Moriyama H, Eskridge KM, Moriyama EN, Somerville GA. Reductive evolution and the loss of PDC/PAS domains from the genus Staphylococcus. BMC Genomics. 2013;14:524.
  • Natarajan C, Inoguchi N, Weber RE, Fago A, Moriyama H, Storz JF. Epistasis among adaptive mutations in deer mouse hemoglobin. Science. 2013;340(6138):1324-7.
  • Li Y, Hassan YI, Moriyama H, Zempleni J. Holocarboxylase synthetase interacts physically with euchromatic histone-lysine N-methyltransferase, linking histone biotinylation with methylation events. J Nutr Biochem. 2013: 24, 1446-52.
  • Inoguchi N, Oshlo JR, Natarajan C, Weber RE, Fago A, Storz JF, Moriyama H. Deer mouse hemoglobin exhibits a lowered oxygen affinity owing to mobility of the E helix. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013;69(Pt 4):393-8. PMCID: 3614163.
  • Hoshi M, Moriyama H, Matsumoto M. Structure of acrosome reaction-inducing substance in the jelly coat of starfish eggs: a mini review. Biochem Biophys Res Commun. 2012;425(3):595-8.
  • Grispo MT, Natarajan C, Projecto-Garcia J, Moriyama H, Weber RE, Storz JF. Gene duplication and the evolution of hemoglobin isoform differentiation in birds. J Biol Chem. 2012;287(45):37647-58. PMCID: 3488042.
  • Wakabayashi-Ito N, Doherty OM, Moriyama H, Breakefield XO, Gusella JF, O'Donnell JM, Ito N. Dtorsin, the Drosophila ortholog of the early-onset dystonia TOR1A (DYT1), plays a novel role in dopamine metabolism. PLoS One. 2011;6(10):e26183. PMCID: 3192163.
  • Storz JF, Hoffmann FG, Opazo JC, Sanger TJ, Moriyama H. Developmental regulation of hemoglobin synthesis in the green anole lizard Anolis carolinensis. The Journal of experimental biology. 2011;214(Pt 4):575-81. PMCID: 3027470.
  • Natarajan C, Jiang X, Fago A, Weber RE, Moriyama H, Storz JF. Expression and purification of recombinant hemoglobin in Escherichia coli. PLoS One. 2011;6(5):e20176. PMCID: 3098879.
  • Naruse M, Ishikawa R, Sakaya H, Moriyama H, Hoshi M, Matsumoto M. Novel conserved structural domains of acrosome reaction-inducing substance are widespread in invertebrates. Mol Reprod Dev. 2011;78(1):57-66.
  • Badalucco L, Poudel I, Yamanishi M, Natarajan C, Moriyama H. Crystallization of Chlorella deoxyuridine triphosphatase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67(Pt 12):1599-602. PMCID: 3232149.
  • Zhang H, Tully DC, Zhang T, Moriyama H, Thompson J, Wood C. Molecular determinants of HIV-1 subtype C coreceptor transition from R5 to R5X4. Virology. 2010;407(1):68-79.
  • Storz JF, Runck AM, Moriyama H, Weber RE, Fago A. Genetic differences in hemoglobin function between highland and lowland deer mice. J Exp Biol. 2010;213(Pt 15):2565-74.
  • Opiyo SO, Pardy RL, Moriyama H, Moriyama EN. Evolution of the Kdo2-lipid A biosynthesis in bacteria. BMC Evol Biol. 2010;10:362.
  • Hassan YI, Moriyama H, Zempleni J. The polypeptide Syn67 interacts physically with human holocarboxylase synthetase, but is not a target for biotinylation. Arch Biochem Biophys. 2010;495:35-41.
  • Campbell KL, Storz JF, Signore AV, Moriyama H, Catania KC, Payson AP, Bonaventura J, Stetefeld J, Weber RE. Molecular basis of a novel adaptation to hypoxic-hypercapnia in a strictly fossorial mole. BMC Evol Biol. 2010;10(1):214.
  • Storz JF, Runck AM, Sabatino SJ, Kelly JK, Ferrand N, Moriyama H, Weber RE, Fago A. Evolutionary and functional insights into the mechanism underlying high-altitude adaptation of deer mouse hemoglobin. Proc Natl Acad Sci U S A. 2009;106(34):14450-5.
  • Runck AM, Moriyama H, Storz JF. Evolution of duplicated beta-globin genes and the structural basis of hemoglobin isoform differentiation in Mus. Mol Biol Evol. 2009;26(11):2521-32.
  • Homma K, Moriyama H. Crystallization and crystal-packing studies of Chlorella virus deoxyuridine triphosphatase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65(Pt 10):1030-4.
  • Hassan YI, Moriyama H, Olsen LJ, Bi X, Zempleni J. N- and C-terminal domains in human holocarboxylase synthetase participate in substrate recognition. Mol Genet Metab. 2009;96(4):183-8.
  • Storz JF, Moriyama H. Mechanisms of hemoglobin adaptation to high altitude hypoxia. High Alt Med Biol. 2008;9(2):148-57.
  • Storz JF, Hoffmann FG, Opazo JC, Moriyama H. Adaptive Functional Divergence Among Triplicated {alpha}-Globin Genes in Rodents. Genetics. 2008;178(3):1623-38.
  • Nakachi M, Matsumoto M, Terry PM, Cerny RL, Moriyama H. Identification of guanylate cyclases and related signaling proteins in sperm tail from sea stars by mass spectrometry. Marine biotechnology (New York, NY. 2008;10(5):564-71.
  • Nakachi M, Hoshi M, Matsumoto M, Moriyama H. Conserved sequences of sperm-activating peptide and its receptor throughout evolution, despite speciation in the sea star Asterias amurensis and closely related species. Zygote. 2008;16(3):229-37.
  • Casas-Mollano JA, Jeong BR, Xu J, Moriyama H, Cerutti H. The MUT9p kinase phosphorylates histone H3 threonine 3 and is necessary for heritable epigenetic silencing in Chlamydomonas. Proc Natl Acad Sci U S A. 2008;105(17):6486-91.
  • Storz JF, Sabatino SJ, Hoffmann FG, Gering EJ, Moriyama H, Ferrand N, Monteiro B, Nachman MW. The molecular basis of high-altitude adaptation in deer mice. PLoS Genet. 2007;3(3):e45.
  • Bajaj M, Moriyama H. Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007;63(Pt 5):409-11.
  • Xu W, Ahmed S, Moriyama H, Chollet R. The Importance of the Strictly Conserved, C-terminal Glycine Residue in Phosphoenolpyruvate Carboxylase for Overall Catalysis: MUTAGENESIS AND TRUNCATION OF GLY-961 IN THE SORGHUM C4 LEAF ISOFORM. J Biol Chem. 2006;281(25):17238-45.
  • Nakachi M, Moriyama H, Hoshi M, Matsumoto M. Acrosome reaction is subfamily specific in sea star fertilization. Dev Biol. 2006;298:597-604.
  • Alvarez-Venegas R, Sadder M, Hlavacka A, Baluska F, Xia Y, Lu G, Firsov A, Sarath G, Moriyama H, Dubrovsky JG, Avramova Z. The Arabidopsis homolog of trithorax, ATX1, binds phosphatidylinositol 5-phosphate, and the two regulate a common set of target genes. Proc Natl Acad Sci U S A. 2006;103(15):6049-54.
  • Abdelnoor RV, Christensen AC, Mohammed S, Munoz-Castillo B, Moriyama H, Mackenzie SA. Mitochondrial genome dynamics in plants and animals: convergent gene fusions of a MutS homologue. J Mol Evol. 2006;63(2):165-73.
  • Zhang Y, Moriyama H, Homma K, Van Etten JL. Chlorella virus-encoded deoxyuridine triphosphatases exhibit different temperature optima. J Virol. 2005;79(15):9945-53.
  • Sugiura I, Sasaki C, Hasegawa T, Kohno T, Sugio S, Moriyama H, Kasai M, Matsuzaki T. Structure of human translin at 2.2 A resolution. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 4):674-9.
  • Kadonosono T, Chatani E, Hayashi R, Moriyama H, Ueki T. Minimization of cavity size ensures protein stability and folding: structures of Phe46-replaced bovine pancreatic RNase A. Biochemistry. 2003;42(36):10651-8.
  • Kumasaka T, Yamamoto M, Yamashita E, Moriyama H, Ueki T. Trichromatic Concept Optimizes MAD Experiments in Synchrotron X-Ray Crystallography. Structure (Camb). 2002;10(9):1205.
  • Chatani E, Hayashi R, Moriyama H, Ueki T. Conformational strictness required for maximum activity and stability of bovine pancreatic ribonuclease A as revealed by crystallographic study of three Phe120 mutants at 1.4 A resolution. Protein Sci. 2002;11(1):72-81.
  • Qu C, Akanuma S, Tanaka N, Moriyama H, Oshima T. Design, X-ray crystallography, molecular modelling and thermal stability studies of mutant enzymes at site 172 of 3-isopropylmalate dehydrogenase from Thermus thermophilus. Acta Crystallogr D Biol Crystallogr. 2001;57(Pt 2):225-32.
  • Numata K, Hayashi-Iwasaki Y, Kawaguchi J, Sakurai M, Moriyama H, Tanaka N, Oshima T. Thermostabilization of a chimeric enzyme by residue substitutions: four amino acid residues in loop regions are responsible for the thermostability of Thermus thermophilus isopropylmalate dehydrogenase. Biochim Biophys Acta. 2001;1545(1-2):174-83.
  • Hirose R, Suzuki T, Moriyama H, Sato T, Yamagishi A, Oshima T, Tanaka N. Crystal structures of mutants of Thermus thermophilus IPMDH adapted to low temperatures. Protein Eng. 2001;14(2):81-4.
  • Yamamoto M, Kumasaka T, Yamashita E, Moriyama H, Sato M, Ueki T. Prospects for X-ray crystal structure analysis of selenoproteins with Spring-8 synchrotron radiation. J Helth Sci. 2000(46):426-9.
  • Mitsuhashi S, Mizushima T, Yamashita E, Yamamoto M, Kumasaka T, Moriyama H, Ueki T, Miyachi S, Tsukihara T. X-ray structure of beta-carbonic anhydrase from the red alga, Porphyridium purpureum, reveals a novel catalytic site for CO(2) hydration. J Biol Chem. 2000;275(8):5521-6.
  • Lu N, Moriyama H, Nakamura S, Sato T, Tanaka N. Crystallization and initial X-ray analysis of alkaline xylanase. Acta Crystallogr D Biol Crystallogr. 2000;56:464-5.
  • Hoshi M, Nishigaki T, Kawamura M, Ikeda M, Gunaratne J, Ueno S, Ogiso M, Moriyama H, Matsumoto M. Acrosome reaction in starfish: signal molecules in the jelly coat and their receptors. Zygote. 2000;8 Suppl 1:S26-7.
  • Hori T, Moriyama H, Kawaguchi J, Hayashi-Iwasaki Y, Oshima T, Tanaka N. The initial step of the thermal unfolding of 3-isopropylmalate dehydrogenase detected by the temperature-jump Laue method. Protein Eng. 2000;13(8):527-33.
  • Mechanisms of Biological Adaptation; Atomic Description of Biological Macromolecules
  • Ph.D. Osaka University
  • M.S. Kumamoto Institute of Technology
  • B.S. Kumamoto Institute of Technology