Qingsheng Li

Associate Professor Profile Image
Associate Professor Biological Sciences qli4@unl.edu 402-472-5922 Morrison Center 305

Research Interests

My research is focused on studying HIV-1 pathogenesis, transmission and prevention using single cell in situ analyses and in vivo SIV-rhesus macaque and humanized-BLT (bone marrow liver and thymus) mouse models of HIV-1 infection. I have published extensively on HIV/SIV transmission, pathogenesis and prevention, including one paper in Science and two papers in Nature as the leading author.

Book Chapters

  • Li Q.(2014). T cell based HIV-1 vaccine in the era of broadly neutralizing antibody (in Chinese). In Wang YC (Ed.) HIV Vaccine Development and Evaluation.
  • Li Q and C. Wood. (2014). Humanized-mouse versus non-human primate models of HIV-1 infection, In Poluektova LY, Cracia-Martinez JV., Koyanagi Y, Manz MG. and Tager AM (Ed.). Humanized Mice for HIV Research. Springer.
  • Li Q.(2014). Collagen Deposition and Fibrosis in the Lymphatic Tissues of HIV-1, in Thomas Hope, Douglas D. Richman, Mario Stevenson(Ed.). Encyclopedia of AIDS Encyclopedia of AIDS. Springer.

Recent Publications

  • Li Q, Tso FY, Kang G, Lu W, Li Y, Fan W, Yuan Z, Destache CJ, Wood C. 2015. Early Initiation of Antiretroviral Therapy Can Functionally Control Productive HIV-1 Infection in Humanized-BLT Mice. J Acquir Immune Defic Syndr. 15; 69 (5):519-27. PMCID: 4503390.
  • Li Y, Kang G, Duan L, Lu W, Katze MG, Lewis MG, Haase AT, Li Q. 2015. SIV Infection of Lung Macrophages. PLoS One. 10:e0125500.
  • Abdulhaqq, SA., C. Zorrilla, G. Kang, X. Yin, V. Tamayo, KE. Seaton, J. Joseph, S. Garced, GD. Tomara, KA. Linn, AS. Foulkes, L Azzoni, M. VerMilyea, C. Coutifaris, AV. Kossenkov, LC. Showe, E. Kraiselburd, Q. Li, LJ. Montaner. HIV-1 Negative Female Sex Workers Sustain High Cervical IFNε, and Low Immune Activation and Expression of HIV-1 Required Host Genes, Mucosal Immunology, accepted for publication on Sept 30, 2015.
  • Yukl SA, Shergill AK, Girling V, Li Q, Killian M, Epling L, Li P, Kaiser P, Haase A, Havlir DV, McQuaid K, Sinclair E, Wong JK. 2015. Site-specific differences in T cell frequencies and phenotypes in the blood and gut of HIV-uninfected and ART-treated HIV+ adults. PLoS One. 10:e0121290.
  • Billingsley JM, Rajakumar PA, Connole MA, Salisch NC, Adnan S, Kuzmichev YV, Hong HS, Reeves RK, Kang HJ, Li W, Li Q, Haase AT, Johnson RP. 2015. Characterization of CD8+ T cell differentiation following SIV Delta nef vaccination by transcription factor expression profiling. PLoS Pathog 11:e1004740.
  • Haase AT, Rakasz E, Schultz-Darken N, Nephew K, Weisgrau KL, Reilly CS, Li Q, Southern PJ, Rothenberger M, Peterson ML, Schlievert PM. 2015. Glycerol Monolaurate Microbicide Protection against Repeat High-Dose SIV Vaginal Challenge. PLoS One 10:e0129465. 2014
  • Demers, A., G. Kang, F. Ma, W. Lu, Z. Yuan, Y. Li, M. Lewis, E. N. Kraiselburd, L. Montaner, and Q. Li. 2014.The mucosal expression pattern of interferon-epsilon in rhesus macaques. J Leukocyte Biology, 96:1101-1107
  • Lu W, F. Ma, A. Tchourbanov, Y., Li, G. Kang, Z. Yuan, D. Wang, C. Zhang, J. Xu, M. Lewis, Q. Li. 2014. Virus-host mucosal interactions during early SIV rectal transmission. Virology, 464-465:406-414. PMID: 25128762
  • Wang, L. X., G. Kang, P. Kumar, W. Lu, Y. Li, Y. Zhou, Q. Li, and C. Wood. Humanized-BLT mouse  model of Kaposi's sarcoma-associated herpesvirus infection. Proc Natl Acad Sci U S A, 2014,  111:3146-51.  PMCID: PMC3939909   (Corresponding author).
  • Wang, N., Y. Li, W. Niu, M. Sun, R. Cerny, Q. Li, and J. Guo. Construction of a Live-Attenuated HIV-1 Vaccine through Genetic Code Expansion. Angewandte  Chemie  International. 2014, 5;53 (19):4867-71. PMID: 24715496  (Corresponding author) (Nat Chem commented this paper by  Lin, S., and P. R. Chen. Virus engineering: Fighting HIV at its own game. Nat Chem 2014 6:566-568)
  • Wang, Z., A. Zhang, Y. Wan, X. Liu, C. Qiu, X. Xi, Y. Ren, J. Wang, Y. Dong, M. Bao, L. Li, M. Zhou, S. Yuan, J. Sun, Z. Zhu, L. Chen, Q. Li, Z. Zhang, X. Zhang, S. Lu, P. C. Doherty, K. Kedzierska, and J. Xu. 2014. Early hypercytokinemia is associated with interferon-induced transmembrane protein-3 dysfunction and predictive of fatal H7N9 infection. Proc Natl Acad Sci U S A, 2014, 111:769-774.
  • Demers, A., G. Kang, F. Ma, W. Lu, Z. Yuan, Y. Li, M. Lewis, E. N. Kraiselburd, L. Montaner, and Q. Li.  The mucosal expression pattern of interferon-epsilon in rhesus macaques. J Leukoc Biol , 2014. 96:1101-1107. (Corresponding author). 
  • Lu W, F. Ma, A. Tchourbanov, Y.  Li, G. Kang,  Z. Yuan, D. Wang, C. Zhang, J. Xu, M. Lewis, Q. Li.    Virus–host mucosal interactions during early SIV rectal transmission. Virology, 2014  464–465:406-414.  PMID: 25128762. Corresponding author.
  • Li, Q., M. Zeng, L. Duan, J. E. Voss, A. J. Smith, S. Pambuccian, L. Shang, S. Wietgrefe, P. J. Southern, C. S. Reilly, P. J. Skinner, M. L. Zupancic, J. V. Carlis, M. Piatak, D. Waterman, R. K. Reeves, K. Masek-Hammerman, C. A. Derdeyn, M. D. Alpert, D. T. Evans, H. Kohler, S. Müller, J. Robinson, J. D. Lifson, D. R. Burton, R. P. Johnson, and A. T. Haase. Live Simian Immunodeficiency Virus Vaccine Correlate of Protection: Local Antibody Production and Concentration on the Path of Virus Entry. The Journal of Immunology. 2014, 193:3113-3125. (cover article)
  • Shaheed A., M. Martinez, G. Kang, A. Foulkes, I. V. Rodriguez,  S.M. Nichols, M. Hunter, C. Sariol, L.A. Ruiz, B. Ross, X. Yin, D. Speicher, A. Haase, P.A. Marx, Q. Li, E. K., and L.J. Montaner. 2014. Serial Cervicovaginal Exposures With Replication-Deficient SIVsm Induce Higher Dendritic Cell (pDC) and CD4+ T-Cell Infiltrates Not Associated With Prevention but a More Severe SIVmac251 Infection of Rhesus Macaques. J. AIDS 65:405-413 410. PMID: 24226059.
  • Smith, A. J., S. W. Wietgrefe, L. Shang, C. S. Reilly, P. J. Southern, K. E. Perkey, L. Duan, H. Kohler, S. Müller, J. Robinson, J. V. Carlis, Q. Li, R. P. Johnson, and A. T. Haase. 2014. Live Simian Immunodeficiency Virus Vaccine Correlate of Protection: Immune Complex–Inhibitory Fc Receptor Interactions That Reduce Target Cell Availability. The Journal of Immunology. 2014 193:3126-3133.
  • Chandirasegaran M; A Gangaplara; T Jia; C Elowsky; G Kang; JJ Riethoven; Q Li; Y Zhou; J Reddy. Direct staining with major histocompatibility complex class II dextramers permits detection of antigen-specific, autoreactive CD4 T cells in situ. Plus One, 2014  PLoS ONE 9:e87519.
  • Gibbs, A., T. Hirbod, Q. Li, K. Bohman, T. B. Ball, F. A. Plummer, R. Kaul, J. Kimani, K. Broliden, and A. Tjernlund.  Presence of CD8+ T Cells in the Ectocervical Mucosa Correlates with Genital Viral Shedding in HIV-Infected Women despite a Low Prevalence of HIV RNA-Expressing Cells in the Tissue. J Immunol 2014, 192:3947-57.
  • Wang, N., Y. Li, W. Niu, M. Sun, R. Cerny, Q. Li, and J. Guo. 2014. Construction of a Live-Attenuated HIV-1 Vaccine through Genetic Code Expansion. Angewandte  Chemie  International  2014,  Article first published online: 8 APR 2014.
  • Massilamany, C., A. Gangaplara, T. Jia, C. Elowsky, Q. Li, Y. Zhou, and J. Reddy. In situ detection of autoreactive CD4 T cells in brain and heart using major histocompatibility complex class II dextramers.  Journal of Visualized Experiments 2014 e51679.
  • Chandirasegaran M; A Gangaplara; T Jia; C Elowsky; G Kang; JJ Riethoven; Q Li; Y Zhou; J Reddy. Direct staining with major histocompatibility complex class II dextramers permits detection of antigen-specific, autoreactive CD4 T cells in situ. Plus One, 2014  PLoS ONE 9:e87519.
  • Wang LX, G Kang, P Kumar, W Lu, Y Li, Y Zhou, Q Li, C Wood. Humanized-BLT Mouse Model of Kaposi's Sarcoma-Associated Herpesvirus Infection.  Proc Natl Acad Sci U S A 2014,  111:3146-51.
  • Wang, Z., A. Zhang, Y. Wan, X. Liu, C. Qiu, X. Xi, Y. Ren, J. Wang, Y. Dong, M. Bao, L. Li, M. Zhou, S. Yuan, J. Sun, Z. Zhu, L. Chen, Q. Li, Z. Zhang, X. Zhang, S. Lu, P. C. Doherty, K. Kedzierska, and J. Xu. 2014. Early hypercytokinemia is associated with interferon-induced transmembrane protein-3 dysfunction and predictive of fatal H7N9 infection. Proc. Natl. Acad. Sci. U.S.A, 2014 111:769-774.
  • Abdulhaqq, S. A., M. I. Martinez, G. Kang, A. S. Foulkes, I. V. Rodriguez, S. M. Nichols, M. Hunter, C. A. Sariol, L. A. Ruiz, B. N. Ross, X. Yin, D. W. Speicher, A. T. Haase, P. A. Marx, Q. Li, E. N. Kraiselburd, and L. J. Montaner. Serial Cervicovaginal exposures with Replication-deficient SIVsm induce higher Dendritic Cell (pDC) and CD4+ T-Cell Infiltrates not associated with prevention but a More Severe SIVmac251 Infection of Rhesus Macaques. Journal of Acquired Immune Deficiency Syndromes. 2014, 65:405-413.
  • Bokhari, S., R. Hegde, SM. Bokhari, R. Hegde, S. Callen, H. Yao, Adany, Q. Li, Z. Li, D. Pinson, H-W. Yeh, P. Cheney, and S. Buch . Morphine Potentiates Neuropathogenesis of SIV Infection in Rhesus Macaques. Journal of Neuroimmune Pharmacology . 2011, 6(4): 626–639, PMID 21431470.
  • Patterson LJ, Kuate S, Daltabuit-Test M, Li Q, Xiao P, McKinnon K, Dipasquale J, Cristillo A, Venzon D, Haase A, Robert-Guroff M. Replicating Adenovirus-Simian Immunodeficiency Virus (SIV) Vectors Efficiently Prime SIV-Specific Systemic and Mucosal Immune Responses by Targeting Myeloid Dendritic Cells and Persisting in Rectal Macrophages, Regardless of Immunization Route. Clin Vaccine Immunol. 2012,  19(5):629-37. PMID:  22441384.
  • Reeves RK, TI Evans, J Gillis, FE Wong, G Kang, Q Li, and RP Johnson. SIV infection induces accumulation of plasmacytoid dendritic cells in the gut mucosa. J. Infect. Dis.  2012, 206(9):1462-8. PMID: 22711907.
  • Li Q, Wood C. Challenges and counter challenges in HIV/AIDS. Chin Med J (Engl). 2010 Dec;123(23):3379-80. PMID:22166517.
  • Smith A. J., Q. Li, S.W. Wietgrefe, et al. Host Genes Associated with HIV-1 Replication in Lymphatic Tissue. J. Immunol. 2010,185:5417-24.
  • García A, N.B. Siddappa, Q. Li, et al. AIDS and optic neuritis in a rhesus monkey infected with the R5 clade C SHIV-1157ipd3N4. J Med Primatol. 2010, 39:356–360, PMID- 20412378
  • Yukl, SA.l, S. Gianella,.E. Sinclair, L. Epling, Q. Li, et al. Differences in HIV Burden and Immune Activation within the Gut of HIV Positive Patients Receiving Suppressive Antiretroviral Therapy. J. Infect. Dis. 2010, 202:1553–1561.
  • Li, Q., J.D. Estes, P.M. Schlievert, et al. Glycerol monolaurate prevents mucosal SIV transmission. Nature 2009 458: 1034-1038. PMCID: PMC2785041
  • Li, Q., P.J. Skinner, S.-J. Ha, et al. Visualizing antigen-specific and infected cells in situ predicts outcomes in early viral infection. Science 2009 323: 1726-1729. PMCID: PMC2753492
  • Bosinger, SE., Q. Li, SN. Gordon, et al. Global genomic analysis reveals rapid control of a robust innate response in SIV-infected sooty mangabeys. J. Clin. Invest. 2009, 119:3556–3572. PMCID:PMC2786806.
  • Li, Q., A.J. Smith, T.W. Schacker, J.V. Carlis, L. Duan, C.S. Reilly, and A.T. Haase. Microarray analysis of lymphatic tissue reveals stage-specific, gene-expression signatures in HIV-1 infection. J. Immunol. 2009, 183: 1975–1982.
  • Li Q, J. Skinner P, Duan L, Haase AT. A technique to simultaneously visualize virus-specific CD8+ T Cells and Virus-Infected Cells In situ. J. Vis. Exp.2009 Aug 13;(30)
  • Li, Q., J.D. Estes, L. Duan, J. Jessurun, S. Pambuccian, C. Forster, S. Wietgrefe, M. Zupancic, T. Schacker, C. Reilly, J.V. Carlis, and A.T. Haase. Simian immunodeficiency virus-induced intestinal cell apoptosis is the underlying mechanism of the regenerative enteropathy of early infection. J. Infect. Dis.2008, 197:420-429.
  • Weiler, A.M., Q. Li, L. Duan, et al. Genital ulcers facilitate rapid viral entry and dissemination following intravaginal inoculation with cell-associated simian immunodeficiency virus SIVmac239. J. Virol. 2008 82: 4154-4158. PMCID PMC2292998
  • Estes, J.D., Q. Li, M.R. Reynolds, et al. Premature induction of an immunosuppressive T regulatory response in acute SIV infection. J. Infect. Dis.2006, 193:703-712.
  • Li, Q., L. Duan, J.D. Estes, et al. Peak SIV replication in “resting” memory CD4+ T cells depletes gut lamina propria CD4+ T cells. Nature 2005, 434:1148-1152.
  • Miller, C.J., Q. Li, K. Abel, et al. Propagation and dissemination of infection after vaginal transmission of SIV. J. Virol. 2005 79: 9217-9227
  • Li, Q., J.D. Lifson, L. Duan, et al. Potential roles for follicular dendritic cell associated osteopontin in lymphoid follicle pathology and repair and B cell regulation in HIV-1 and SIV infections. J. Infect. Dis. 2005, 192:1269-1276.
  • Zhang, Z.-Q., S.W. Wietgrefe, Q. Li, et al. Roles of substrate availability and infection of resting and activated CD4+ T cells in transmission and acute simian immunodeficiency virus infection. Proc. Natl. Acad. Sci. U.S.A. 2004, 101:5640-5645.
  • Li, Q., K.G. Mansfield, A.A. Lackner, et al. Quantitative image analysis of simian immunodeficiency virus replication in macrophages co-infected with Mycobacterium avium complex. J. Infect. Dis. 2000, 181:867-871.
Specialization:
  • HIV Transmission, Vaccine, & Prevention
News:
Education:
  • Ph.D., Beijing Univeristy Medical School
  • Master Med. Sc., Shanxi Medical University
  • Medicine, Datong University Medicial School
  • M. SS, Univeristy of St. Thomas